Fig. 6

Expression of MHC isoforms in skeletal muscle. The soleus muscle was isolated in WT and DKO mice to estimate the expression of MHC isoforms by immunofluorescence. MHC I and MHC IIa were stained with blue and red, respectively. Muscle fibres negative for MHC I and MHC IIa were represented as a combination of type IIb and IIx fibres. The bar graph shows the percentage of each MHC isoform. MHC, myosin heavy chain. n = 6. Scale bar, 100 μm