Figure 2

Detection of apoptotic cells by fluorescence microscopy. TheTUNEL assay with fluorescein as the tag was used to stain the nucleiof apoptotic cells in frozen cardiac sections. (A) Control heart(containing essentially no TUNEL-positive cells in this field) and(B) a fluorescent micrograph of a representative field from a failingheart (DCM) containing a higher number of apoptotic nuclei. Micrographswere taken with 40X objective and reduced for reproduction.