Figure 5

Immunofluorescence microscopy of wild-type and Tyr117 mutant mSlc10a2. COS-7 cells transiently expressing T7-tagged mSlc10a2 proteins were incubated with anti-T7 antibody under nonpermeabilized (left panel) or permeabilized (right panel) conditions, followed by fluorescein-conjugated secondary antibody. The merged image of mSlc10a2 immunofluorescence (green) and nuclear staining (blue) is shown.